Latex allergies - standardization of the allergen quantification in products and air samples obtained from the workplaces and the prevention of the hypersensitivity reaction due to latex

Status:

completed 12/2001

Aims:

Aim of this project was the development of standardized methods to determine the most important latex allergens in diagnostics, products of latex and in dust samples of work places.

Activities/Methods:

Determination of latex specific IgE; cloning and expression of recombinant allergens as fusionproteins with maltosebinding protein or with histidin-tag, purification of recombinant proteins due to affinity chromatography. Enrichment of monoclonal antibodies by in vitro production in a modular minifermenter and affinity purification, optimizing of two sided immunoassays for quantification of Hev b 1 and Hev b 6.01, inhibition test to quantify total latex allergen content, quantification of Hev b 5 by using inhibition assay with Hev b 5-specific IgE containing patients sera.

Results:

Nine clones of latex allergens are available at the BGFA: rHev b 1, rHev b 2, rHev b 3, rHev b 5, rHev b 6.01, rHev b 6.02, rHev b 6.03, rHev b 8, rHev b 10. Methods for determination of three of them (Hev b 1, Hev b 6.01 and Hev b 5) are available so far. More than 80% of tested latex sera of health care workers reacted to rHev b 5. In collaboration with industry latex-specific IgE-tests (CAP) were improved by addition of rHev b 5.

Publications:

Baur, X.; Chen, Z.: Summary of appropriate measures to prevent natural rubber latex allergy. Allergologie International 48 (1999), S. 31-36

Chardin, H.; Chen, Z.; Raulf-Heimsoth, M.; Mayer, C.; Senechal, H.; Desvaux, F.X. ; Peltre, G.: Identification of Hev b1 in natural latex mattresses. Int. Arch. Allergy Immunol. 121 (2000), S. 211-214

Chardin, H.; Chen, Z.; Raulf-Heimsoth, M.; Mayer, C.; Senechal, H.; Desvaux, F.X.; Peltre, G.: Detection and 2D gel characterization of two major latex allergens in various latex extracts. Allergy 54 (2001), S. 127-128

Chen, Z.: Impact of isolated and recombinant allergens in diagnosis of latex allergy. In: Booklet Pharmacia Symposium 'Can tests with different allergen components provide new clinical information?'. Lisbon, Portugal 2000, S. 4-5

Chen, Z.; Baur, X.: Naturlatexallergengehalt in Gummiartikeln und in der Raumluft von Krankenhäusern und Arztpraxen. Allergologie 22 (1999), S. 598-605

Chen, Z.; Düser, M.; Flagge, A.; Maryska, S.; Sander, I.; Raulf-Heimsoth, M.; Baur, X.: Identification and Characterization of Cross-Reactive Natural Rubber Latex and Ficus benjamina Allergens. Int. Arch. Allergy Immunol. 123 (2000), S. 291-298

Chen, Z.; Rihs, H.P.; Slater, J.E.; Paupore, E.J.; Schneider, E.M.; Baur, X.: The absence of Hev b 5 in capture antigen may cause false-negative results in serologic assays for latex-specific IgE antibodies. J. Allergy Clin. Immunol.105 (2000), S. 838

Cremer, R.; Chen, Z.; Blaker, F.; Baur, X.: Pattern of IgE antibodies against latex allergens in children with spina bifida. Eur. J. Pediatr. Surg. 9 (Suppl 1) (1999), S. 49-50

Cremer, R.; Chen, Z.; Rihs, H.P.; Baur, X.: Naturlatexallergie: Von der Beschreibung des Phänomens zur Epitopanalyse. Monatsschrift Kinderheilkunde 147 (1999), S. 544-551

Doekes, G.; van Niftrik, M.; Portengen, L.; Tomazic-Jezic, V.J. ; Chen, Z.; Nij, E.I.T.; de Wind, S.; Heederik, D.J.: Measurement of Latex Proteins in Low-Volume Airborne Samples and Glove Extracts with a Sandwich EIA Using Polyclonal Rabbit Anti-Latex IgG. J. Allergy Clin. Immunol. 107 (2001), S. A438

Korn, M.; Chen, Z.; Sander, I. ; Maryska, S.; Raulf-Heimsoth, M.: Gepuderte Kofferdamgummis als Ursache fortgesetzter obstruktiver Ventilationsstörungen bei Zahnarzthelferinnen. Zbl. Arbeitsmed. 51 (2001), S. 181

Lundberg, M.; Chen, Z.; Rihs, H.P.; Wrangsjo, K.: Recombinant spiked allergen extract. Allergy 56 (2001), S. 794-795

Raulf-Heimsoth, M.; Chen, Z.; Rihs, H.; Rozynek, P.: T- und B-Zellepitope im Latexallergen Hev b 6.01 (Prohevein). Allergol. J. 10 (2001), S. 25

Raulf-Heimsoth, M.; Rihs, H.P.; Rozynek, P.; Baur, X.; Chen, Z.: Recombinant latex allergens rHev b 6. 01, rHev b 6.02, rHev b 6.03 induced different T cell reactivity. J. Allergy Clin. Immunol. 107 (2001), S. 381

Raulf-Heimsoth, M.; Sander, I.; Chen, Z.; Borowitzki, G.; Diewald, K.; van Kampen, V.; Baur, X.: Development of a monoclonal antibody-based sandwich ELISA for detection of the latex allergen Hev b 1. Int. Arch. Allergy Immunol. 123 (2000), S. 236-241

Raulf-Heimsoth, M.; Sander, I.; Diewald, K.; Chen, Z.; Borowitzki, G.; van Kampen, V.; Baur, X.: Development of a monoclonal antibody based sandwich ELISA for the detection of the latex allergen Hev b1. Am. J. Resp. Crit. Care Med. 159 (1999), S. A233

Rihs, H.P.; Chen, Z.; Rozynek, P.; Baur, X.; Lundberg, M.; Cremer, R.: PCR-based cloning, isolation, and IgE-binding properties of recombinant latex profilin (rHev b 8). Allergy 55 (2000), S. 712-717

Rihs, H.P.; Chen, Z.; Rozynek, P.; Cremer, R.: Allergenicity of rHev b 10 (manganese-superoxide dismutase). Allergy 56 (2001), S. 85-86

Rihs, H.P.; Chen, Z.; Schumacher, S.; Rozynek, P.; Cremer, R.; Lundberg, M.; Raulf-Heimsoth, M.; Petersen, A.; Baur, X.: Recombinant hev b 1: large-scale production and immunological characterization. Clin. Exp. Allergy 30 (2000), S. 1285-1292

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